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Preparation of Anti-oxidation Bioactive Peptide of Rice Bran Protein

Min Zhang

Open publisher page 4 citations

Abstract

This study screened the most suitable prolease of preparation of bioactive peptide with the indexes of Hydrolysis(DH%) and the eliminating rate of DPPH free radical.In order to find the most suitable zymohydrolysis condition of prolease,effects of concentration of substrate,pH,addition of protease and dissociation-time on the hydrolysis(DH%) and the eliminating rate of DPPH freeradical in hydrolyzing were investigated.Based on the single-factor experiment,the optimum conditions of extraction of bioactive peptide from rice bran by the Box-Behnken response surface methodology design of Design-Expert 7.0 were determined as follows:addition amount of enzyme 13970.82 U/g,dissociation-time 3 h and concentration 4.97%.The results showed that degree of hydrolysis of rice bran protein was 23.67% and the eliminating rate of DPPH free radical could reach 64.26% under the optimum conditions.

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What this paper is about

This study screened the most suitable prolease of preparation of bioactive peptide with the indexes of Hydrolysis(DH%) and the eliminating rate of DPPH free radical.In order to find the most suitable zymohydrolysis condition of prolease,effects of concentration of substrate,pH,addition of protease and dissociation-time on the hydrolysis(DH%) and the eliminating rate of DPPH freeradical in hydrolyzing were investigated.Based on the single-factor experiment,the optimum conditions of extraction of bioactive peptide from rice bran by the Box-Behnken response surface methodology design of Design-Expert 7.0 were determined as follows:addition amount of enzyme 13970.82 U/g,dissociation-time 3 h and concentration 4.97%.The results showed that degree of hydrolysis of rice bran protein was 23.67% and the eliminating rate of DPPH free radical could reach 64.26% under the optimum conditions.

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Available abstract

This study screened the most suitable prolease of preparation of bioactive peptide with the indexes of Hydrolysis(DH%) and the eliminating rate of DPPH free radical.In order to find the most suitable zymohydrolysis condition of prolease,effects of concentration of substrate,pH,addition of protease and dissociation-time on the hydrolysis(DH%) and the eliminating rate of DPPH freeradical in hydrolyzing were investigated.Based on the single-factor experiment,the optimum conditions of extraction of bioactive peptide from rice bran by the Box-Behnken response surface methodology design of Design-Expert 7.0 were determined as follows:addition amount of enzyme 13970.82 U/g,dissociation-time 3 h and concentration 4.97%.The results showed that degree of hydrolysis of rice bran protein was 23.67% and the eliminating rate of DPPH free radical could reach 64.26% under the optimum conditions.

Key concepts: DPPH, Bran, Chemistry, Hydrolysis, Response surface methodology, Protease, Peptide, Dissociation (chemistry)

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