2010•PubMedRequires access

[Construction of recombinant adenovirus containing TK gene and its effect against human liver cancer cells].

Geng-zhen Chen, Hui Hu, Mingyan Xu, Xiaoling Deng

Abstract

OBJECTIVE: To construct a replication-defective adenovirus containing TK gene and investigate the killing effects of TK gene against human liver cancer cells SMMC-7721. METHODS: The recombinant adenovirus ADV-TK was constructed using homologous recombination in the cells. SMMC-7721 cells transfected with recombined adenovirus were exposed to GCV, and the cell viability was measured by MTT assays. RESULTS: The recombinant adenovirus containing TK gene was successfully constructed. Transfection by the recombinant adenovirus ADV-TK and GCV exposure significantly suppressed the growth of SMMC-7721 cells. CONCLUSION: A replication-defective adenovirus containing TK gene has been successfully constructed, and in combination with GCV, the recombinant adenovirus produces significant killing effect against SMMC-7721 cells in vitro.

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Key concepts: Recombinant DNA, Transfection, Molecular biology, Homologous recombination, MTT assay, Genetic enhancement, Adenoviridae, Gene