2004•Zhongguo renshougonghuanbing zazhiRequires access
Immune response induced by DNA vaccine of Trichinella spiralis in BALB/c mice
Han Hua-min
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Abstract
Objective To observe the immune response of mice induced by DNA vaccine of Trichinella spiralis.Methods The BALB/c mice were immunized with the recombinant eukaryotic expression plasmid pcDNA3-TspE1 contained the structural gene encoding a 31 kDa antigen of Trichinella spiralis through two routes: intramuscle injection and gene-gun injection.The anti-Trichinella antibodies in immune sera were detected by indirect fluorescencent antibody test(IFAT)with freezing section of T.spiralis muscle larvae and paraffin section of muscle containing T.spiralis larvae as antigen.The immune sera were also analized by Western blot using T.spiralis muscle larval soluble antigens.Results The results of IFAT showed that the immune sera from mice inoculated by pcDNA3-TspE1 could produce the positive reaction with T.spiralis muscle larvae,the yellow-green fluorescence appeared on the freezing and paraffin section of T.spiralis larvae.Western blot confirmed that sera from mice immunized by pcDNA3-TspE1 were only reacted with the 31kDa antigenic component of T.spiralis larval sobuble antigen,whereas no positive immune reaction was observed between the sera from mice inoculated with pcDNA3 and the soluble antigen of T.spiralis muscle larvae.Conclusion The specific humoral immune response in mice could be produced by DNA vaccine(pcDNA3-TspE1)of Trichinella spiralis.