Study on Pharmacokinetics of Pyroline in Pigs

Normal pigs were given PL(pyroline)iv 30 mg·kg -1 and im 85 mg·kg -1 .The concentrations of PL in porcine plasma was determined by an HPLC method developed in our laboratory.A Waters model 480 instrument was used throughout the experiment.Vanillin was used as the internal standard at absorption wavelength of 220 nm.A mixture of methanol and water (40∶60) was used as the mobile phase with a flow rate of 0.7 ml·min -1 ,and YWG C 18 H 37 as stationary phase.The method is simple,sensitive and available for pharmacokinetics studies.Plasma drug concentration time courses of PL after iv and im administration of PL were both found to be fitted to a two Compartment open model and their pharmacokinetic parameters were respectively as follows:iv,T 1/2α =1 52 min,T 1/2β =66 05 min,AUC=880 07 mg·min·L -1 ,im,T 1/2ka =0 37 min,T 1/2α =3 45 min,T 1/2β =76 87 min,AUC=570 17 mg·min·L -1 ,T p=1 53 min,Cmax=11 34 μg·ml -1 .Results show:after pigs were given PL iv and im administration,PL was absorbed immediately,distributed rapidly and widely,eliminated fast.Moreover,bioavailability factor of PL in im adminstration was low,