Effect of high glucose on endothelial function

Objective To investigate effect of high glucose on the function of endothelial and the underlying mechanisms in human umbilical vascular endothelial cells( HUVECs). Methods The experiment was divided into 4 groups: normal group( NG),low dose group( LG),middle dose group( MG) and high dose group( HG). The concentration of glucose in the culture medium was 5. 5,10,20,30 mmol / L in the 4 groups,respectively. The HUVECs was cultured for 0,24,48 h. At different time point,the cell viability were measured by MTT. The secretary content of nitric oxide( NO) in the supernatant were detected using test kit. The extraction of protein were extracted for Western blot analysis to detect the expression of endothelial nitric oxide synthase( e NOS). Results Compared with normal group at same time point( cultured for 24 h),the cell viability and the content of NO were significantly decreased in LG,MG( P 0. 05). The expression of eNOS in HG were markedly reduced( P 0. 01). Compared with normal group at same time point( cultured for 48 h),the cell viability decreased significantly in HG( P 0. 05). The expression of eNOS were markedly decreased 11. 91,25. 72 and 34. 50% in LG,MG and HG,respectively. A rising trend of cell viability were found in NG,LG and MG,but a descending trend were found in HG within 48 h. Conclusion The cell viability were significantly affected by high glucose. The endothelial dysfunction induced by high glucose may be associated with the reduction of e NOS and NO production.