Application on nucleic acid amplification technigues in blood screening for HBV DNA,HCV RNA/HIV-1 RNA

Objective To investigate and analyze the conditions of seronegative NAT positive donors,and study the feasibility of NAT in blood screening.Methods Seronegative samples were detected by Roche PCR、PCR-CHIP and real-time fluorescence PCR for HBV DNA、HCV RNA and HIV-1 RNA.The positive donors were traced and analyzed by quantitative PCR.Results 22 of 131 174 donations were HBV DNA positive,the HBV DNA positive rate was 1∶5 962,15/22 donations were Anti-HBc positive,the HBV DNA and Anti-HBc positive rate was 1∶8 745,14 donors were traced and 8 follow-up samples seroconverted,1 was conformed HCV RNA positive,the positive rate was 1∶131 174.Conclusion It is necessary to implement high sensitive HBV and HCV NAT detection in blood screening for blood safety.