Study on function of transplanted livers and content of hepatic glycogen in donor livers stored in low temperature

Objective:To study the function of transplanted liver and content of hepatic glycogen in donor livers stored in low temperature.Methods:56 rabbits were divided into liver donor group and liver recipient group.The two groups were further divided into four subgroups,i.e.fasted 24 h group(group A),common feed group(group B),common feed concurrently with intravenous injection of glucose 4 times group(group C) and 6 times group(group D).The livers of donor group were taken away and stored in 4 ℃ for 9 h.By the transplanted livers regained blood supply for 2 h,the samples of blood of recipient groups were collected;the liver functions and content of hepatic glycogen of recipient group were examined.Results:The contents of hepatic glycogen in donor liver from group A was(15.42±2.60)mg/g,group B was(51.83±6.61)mg/g,group C was(63.22±5.84)mg/g and group D was(78.58±8.46)mg/g,respectively,and the differences among four groups were significant(P0.05).The changes of levels of AST,ALT and AKP in blood samples were group Agroup Bgroup Cgroup D and the differences among four groups were significant(P0.05).Conclusion:Before donor liver is taken away,addition supply of hepatic glycogen may be helpful to relieve cold ischemic damage of donor liver and elevate the achievement ratio of liver transplantation.