The experimental study on the relationship between the hepatic glycogen content and the liver transplantation of non-heart-beating donor

Objective To study whether the warm ischemia-reperfusion injury can be alleviated if the hepatic glycogen content were increased before liver transplantation.Methods The male SD rats as donors and recipients were divided into groups A,B and C randomly.In group A,the rats were al- lowed access to solid and water ad libitum;In group B,the rats drank glucose liquor for 4 days prior to liver harvesting;In group C,the rats were intravenously injected with 50% glucose besides the protocol in group B 3-4 h prior to liver harvesting.According to the non-heart-beating time(the non- heart-beating time were set into 60,90,120 and 150 min),the rats in groups A,B and C were divided into 4 subgroups,and the orthotopic liver transplantation was performed and the hepatic glycogen and ATP contents were measured.The one-week survival rate and the serum levels of MDA and SOD in the recipients were determined after liver transplantation.Results The hepatic glycogen and ATP con- tents in groups B and C were more than those in group A significantly(P0.01).The one-week sur- vival rate in subgroups B(60,90,120 min)was 80%,50%,10% and in subgroups C(90,120, 150 min)was 70%,60%,20%,respectively.Some recipients could achieve long-term survival. The serum levels of MDA in groups B and C were lower than in group A,and the levels of SOD in groups B and C were higher than those in group A(P0.05).Conclusion With the increase of the hepatic glycogen content before liver harvesting,the warm ischemia-reperfusion injury of the grafted liver can be alleviated and incidence of the primary non-function of grafted liver can be reduced,and the survival rate of recipients in the non-heart-beating-donor also can be improved after liver transplan- tation.