Comparative studies on serum protein and isozymes of bumper crucian carp(carassius auratus of pengze♀×cyprinus acutidoralis♂) and its parents
Zhao Jun, Miao Cui, Qing Xiu Ning, Xianglin Chen
Abstract
Zhao Jun, Miao Cui, Qing Xiu Ning, Xianglin Chen
Abstract
Three isozymes (EST, ME and SOD) of four tissues (heart, liver, kidney, brain) and serum protein of Bumper crucian carp (Carassius auratus of pengze♀×Cyprinus acutidorsalis♂), its female parent Pengze crucian carp and male parent seap carp (C. acutidorsalis) were analyzed with vertical polyacrylamide gel electrophoresis (7.5%). The result showed that the exectrophoric patterns of isozymes and serum protein of F_1 were the same as those of its female parent and significantly different from its male parent. Thus, F_1 was thought as the progeny of gynogenesis. In the aspects of isozymes and serum protein patterns, no paternal inheritance has been found in mature progeny.
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Three isozymes (EST, ME and SOD) of four tissues (heart, liver, kidney, brain) and serum protein of Bumper crucian carp (Carassius auratus of pengze♀×Cyprinus acutidorsalis♂), its female parent Pengze crucian carp and male parent seap carp (C. acutidorsalis) were analyzed with vertical polyacrylamide gel electrophoresis (7.5%). The result showed that the exectrophoric patterns of isozymes and serum protein of F_1 were the same as those of its female parent and significantly different from its male parent. Thus, F_1 was thought as the progeny of gynogenesis. In the aspects of isozymes and serum protein patterns, no paternal inheritance has been found in mature progeny.
Key concepts: Crucian carp, Carassius auratus, Isozyme, Carp, Cyprinus, Biology, Polyacrylamide gel electrophoresis, Cyprinidae