Comparative Study on the Isozymes of Offspring Developed from Pengze crucian Carp Eggs Activated by Different Sperms and Their Parents
Dong Cheng-wen, Xianglin Chen
Abstract
Dong Cheng-wen, Xianglin Chen
Abstract
The eggs of pengze crucian carp (Carassius auratus of Pengze) were stimulated by pengze crucian carp and sea carp (Cyprinus acutidorsalis Wang) sperms, respectively.The offspring were called PP and PS, respectively. Four isozymes (LDH, EST, SOD, MDH) of three tissues (heart, kidney and liver) and muscle proteins of the offspring and their parents above were investigated used by polyacrylamide gel electrophoresis method. The results showed that the isozymes patterns of PP and PS were almost identical, that means no influence on the offspring coming from different sperms. But differences were found in some isozymes and muscle protein phenotype between the offspring and their maternal parent. That maybe hinted the gynogenesis of pengze crucian carp is different from the traditional gynogenesis of unisexual animals.
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The eggs of pengze crucian carp (Carassius auratus of Pengze) were stimulated by pengze crucian carp and sea carp (Cyprinus acutidorsalis Wang) sperms, respectively.The offspring were called PP and PS, respectively. Four isozymes (LDH, EST, SOD, MDH) of three tissues (heart, kidney and liver) and muscle proteins of the offspring and their parents above were investigated used by polyacrylamide gel electrophoresis method. The results showed that the isozymes patterns of PP and PS were almost identical, that means no influence on the offspring coming from different sperms. But differences were found in some isozymes and muscle protein phenotype between the offspring and their maternal parent. That maybe hinted the gynogenesis of pengze crucian carp is different from the traditional gynogenesis of unisexual animals.
Key concepts: Crucian carp, Carassius auratus, Offspring, Carp, Biology, Isozyme, Cyprinus, Polyacrylamide gel electrophoresis