2000•Journal of Shanghai MedicaRequires access
Rat Mesangial Cell Transfected by Human Transforming Growth Factor β1 Gene Upregulates the Expression of Matrix Metalloproteinase 2
Lin Wen
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Abstract
Purpose To study the matrix metalloproteinase 2(MMP?2) expression on cultrured rat mesangial cells(MsC) transfected by human transforming growth factor β1(TGF?β1) gene. Methods Lipofectin method for transfecting human TGF?β1 gene into MsC;Northern blot analysis for detecting TGF?β1 mRNA level in MsC.MMP?2 mRNA,protein and enzyme activity were determined by Northern blot,Western blot,Zymography assay,respectively. Results The MsC clone (MTG1) overexpressed human TGF?β1 mRNA was successfully prepared and its expression of MMP?2 mRNA,protein and enzyme activity was increased. Results The MsC clone (MTG1) overexpressed human TGF?β1 mRNA was successfully prepared and its expression of MMP?2 mRNA,protein and enzyme activity was increased. Conclusions TGF?β1 might upregulate the expression of MMP?2 and enhance the enzymatic activity of MMP?2 on cultured MsC.It is valuable for explaining the role of TGF?β1 in the development of glomerulsclerosis.