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2000•Journal of Shanghai MedicaRequires access

Expression of MMP-2,9 on Human Hepatoma Cells Transfected with TGF-β_1 Gene

Lin Wen

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Abstract

Purpose To study the expression of MMP?2,9 on human hepatoma cells(SMMC?7721)transfected with TGF?β 1 gene. Methods TGF?β 1 gene transfection was done by electroporation.The expression of TGF?β 1 and MMP?2,9 on transfectants was detected by Northern blot and Western blot analysis. Results Higher mRNA and protein level of MMP?2 were observed in SMMC?7721 cells transfected with TGF?β 1 gene,however,the expression of MMP?9 was not affected by TGF?β 1. Conclusions TGF?β 1 might upregulate the expression of MMP?2 on human hepatoma cells,it is possible that TGF?β 1 is involved in the invasion and metastasis of human hepatoma.

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Purpose To study the expression of MMP?2,9 on human hepatoma cells(SMMC?7721)transfected with TGF?β 1 gene. Methods TGF?β 1 gene transfection was done by electroporation.The expression of TGF?β 1 and MMP?2,9 on transfectants was detected by Northern blot and Western blot analysis. Results Higher mRNA and protein level of MMP?2 were observed in SMMC?7721 cells transfected with TGF?β 1 gene,however,the expression of MMP?9 was not affected by TGF?β 1. Conclusions TGF?β 1 might upregulate the expression of MMP?2 on human hepatoma cells,it is possible that TGF?β 1 is involved in the invasion and metastasis of human hepatoma.

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Available abstract

Purpose To study the expression of MMP?2,9 on human hepatoma cells(SMMC?7721)transfected with TGF?β 1 gene. Methods TGF?β 1 gene transfection was done by electroporation.The expression of TGF?β 1 and MMP?2,9 on transfectants was detected by Northern blot and Western blot analysis. Results Higher mRNA and protein level of MMP?2 were observed in SMMC?7721 cells transfected with TGF?β 1 gene,however,the expression of MMP?9 was not affected by TGF?β 1. Conclusions TGF?β 1 might upregulate the expression of MMP?2 on human hepatoma cells,it is possible that TGF?β 1 is involved in the invasion and metastasis of human hepatoma.

Key concepts: Transfection, Electroporation, Molecular biology, Western blot, Northern blot, Gene expression, Gene, Transforming growth factor

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Expression of MMP-2,9 on Human Hepatoma Cells Transfected with TGF-β_1 Gene — Research Paper | ScholarLens