Expression of MMP-2 and MMP-9 in tissue and serum in human with gastric carcinoma and their clinical significance

Objective To study the relativity of levels of MMP-2 and MMP-9 in serum and cancer tissues got from gastric carcinoma patients,dicuss relationship between them and clinical pathology parameters.Methods 38 gastric carcinoma paraffin embed specimens resected by operation and serum of pre-operation were collected.PV-9000 polymer detection system for immunohistological staining and enzyme-linked immunosorbent assay were used to detect the the expression of MMP-2 and MMP-9 in 38 resected specimens and serum.Results The positive expression of MMP-2 and MMP-9 in gastric carcinoma tissue specimens respectively was 94.7%and 86.8%,and there was significant relationship between the expression of MMP-2 and MMP-9 and the lymph node metastasis,TNM stage(P0.05);there was no significant difference between the patients and the control group of MMP-2 in serum(P0.05);ther was significant difference between the patients and the control group of MMP-9 in serum(P0.05),and there was significant relationship between the expression of MMP-9 in serum and the lymph node metastasis,TNM stage(P0.05,respectively).Conclusion There is high expression of MMP-2 and MMP-9 in gastric carcinoma tissues,and there was significant correlation with metastasis of lymph node and TNM stage.There was relativity of the expression of MMP-9 between the serum and the tissue.