Effect of liposome-mediated c-myc antisense oligodeoxynucleotide on biological behaviors of breast cancer MCF-7cells

Objective:To investigate the effects of liposome (LipfectAMINE TM ,namely LR)-mediated c-myc antisense oligodeoxynucleotide(ASODN) on cell proliferation,c-myc protein expression,and apoptosis of MCF-7 cells. Methods:Cell proliferation was measured by MTT assay. Expression of c-myc protein was measured by immunocytochemistry before and after transfection. Apoptosis of cells was detected by flow cytometry. Results:Transfection of ASODN/LR markedly inhibited the proliferation of MCF-7 cells compared with sense and missense ODN( P 0.01). FCM analysis showed that there appeared an obvious apoptosis peak after transfection. The apoptosis rate was maximum (28.76±2.09)% at 72 h. The immunocytochemistry staining showed that c-myc protein expression was suppressed significantly(21.4±1.16)%. Conclusion:Liposome-mediated c-myc ASODN can evidently inhibit cell proliferation,down-regulate c-myc protein expression,and induce apoptosis of MCF-7 cells.