Induction of Apoptosis by 8-bromo-5-hydroxy-7-methoxychrysin in Acute T Lymphocyte Leukemia Jurkat Cell Line

Objective To investigate the effects of 8-bromo-5-hydroxy-7-methoxychrysin(BrMC) on apoptosis of human acute T lymphocyte leukemia Jurkat cell line.Methods Jurkat cells were cultured in vitro.Flow cytometry(FCM) after FITC labeled annexin V staining was used to determine the percentage of apoptotic cells.Histone/DNA fragments were determined using ELISA assay.The generation of reactive oxygen species(ROS) was analyzed by FCM using the fluorescent probe 2' 7'-dichlorofluoresein diacetate(DCFH-DA).Results BrMC significantly induced increase of the percentage of apoptotic cells(P0.05),and increased level of the histone/DNA fragments in Jurkat cell line(P0.05),in a concentration-dependent manner.BrMC elevated ROS generation level in Jurkat cells(P0.05),in a concentration-dependent manner.N-Acetylcysteine(NAC,10 mmol/L) can block generation of ROS and attenuate induction of apoptosis by BrMC in Jurkat cells(P 0.05).Conclusion BrMC can effectively induce apoptosis of Jurkat cells,and this effect is associated with generation of ROS.