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2015•Zhongguo shouyi xuebaoRequires access

Purification and characterization of acid cellulase fromBacillussubtilis

Li Ma

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Abstract

Bacillus subtilis BY-4,isolated from Tibetan pig,had been cultured in the fermentation medium(containing 4% wheat bran)at 37 ℃ with agitation speed of 220r/min for 48 h.The crude enzyme was purified by hydrophobic interaction chromatography on HiTrap Phenyl HP column and gel filtration chromatography on HiPrep 16/60 Sephacryl S-100 column.A purified endoglucanase was achieved with purification fold of 15.5and a recovery rate of 25.0%.SDS-PAGE and zymogram assay revealed an electrophoretically pure cellulase with apparent molecular weights of 55 000.The optimal values of pH and temperature on purified cellulase response were found to be 4.5and 60℃,respectively.The cellulase can be activated by Mg2+and Zn2+,while inhibited by Mn2+,Co2+and Ag+.

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Bacillus subtilis BY-4,isolated from Tibetan pig,had been cultured in the fermentation medium(containing 4% wheat bran)at 37 ℃ with agitation speed of 220r/min for 48 h.The crude enzyme was purified by hydrophobic interaction chromatography on HiTrap Phenyl HP column and gel filtration chromatography on HiPrep 16/60 Sephacryl S-100 column.A purified endoglucanase was achieved with purification fold of 15.5and a recovery rate of 25.0%.SDS-PAGE and zymogram assay revealed an electrophoretically pure cellulase with apparent molecular weights of 55 000.The optimal values of pH and temperature on purified cellulase response were found to be 4.5and 60℃,respectively.The cellulase can be activated by Mg2+and Zn2+,while inhibited by Mn2+,Co2+and Ag+.

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Available abstract

Bacillus subtilis BY-4,isolated from Tibetan pig,had been cultured in the fermentation medium(containing 4% wheat bran)at 37 ℃ with agitation speed of 220r/min for 48 h.The crude enzyme was purified by hydrophobic interaction chromatography on HiTrap Phenyl HP column and gel filtration chromatography on HiPrep 16/60 Sephacryl S-100 column.A purified endoglucanase was achieved with purification fold of 15.5and a recovery rate of 25.0%.SDS-PAGE and zymogram assay revealed an electrophoretically pure cellulase with apparent molecular weights of 55 000.The optimal values of pH and temperature on purified cellulase response were found to be 4.5and 60℃,respectively.The cellulase can be activated by Mg2+and Zn2+,while inhibited by Mn2+,Co2+and Ag+.

Key concepts: Cellulase, Size-exclusion chromatography, Bran, Biology, Bacillus subtilis, Chromatography, Enzyme, Column chromatography

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