2006•Sichuan Nongye Daxue xuebaoRequires access

Purification and Properties of Cellulase from Bacillus subtilis C-36

Qi Wu

Abstract

A cellulase from Bacillus subtilis C-36 was isolated and purified,by means of ammonium sulfate precipitation,followed by chromatography on Sephadex G-75 and Sephadex G-100.Analysis shows that the purified cellulase is CMCase.SDS-PAGE shows its molecular weight is about 35kD.The optimum temperature is 65℃,and the optimum pH is pH 6.8.The cellulase can be inhibited by Zn 2+,Pb 2+,Fe 3+ and activated by Ba 2+,Fe 2+.Its Km for CMC-Na at 50℃ and pH 6.8 is 0.34g/L.

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Key concepts: Cellulase, Bacillus subtilis, Sephadex, Ammonium sulfate precipitation, Chemistry, Chromatography, Ammonium sulfate, Ammonium