Characteristics comparison of osteoclasts induced by M-CSF and RANKL

The biological characteristics of osteoclasts were induced from mouse bone marrow monocytes and RAW264.7 cells were compared by tartrate resistant acid phosphatase(TRAP) staining and bone resorption pits assaying in this paper.Collecting ICR mouse bone marrow monocytes,osteoclasts were formed by inducation bone marrow monocytes with 25.0 μg/L M-CSF+50.0 μg/L RANKL in vitro,at the same time,osteoclasts were formed by inducation RAW264.7 cells with 100.0 μg/L RANKL.The results showed TRAP positive multinuclear cells induced from bone marrow monocytes were less than from RAW264.7 cells significantly(P0.05),conversely,the area of bone resorption pits was different significantly(P0.05).The cells induced by two methods displayed typical characteristics of osteoclasts,their activity of TRAP was stronger in the cells induced by bone marrow monocytes,but the number and purity of cells induced by RAW264.7 cells were higher.