Culture of osteoclasts induced from bone marrow mesenchymal stem cells in mice

Objective:To investigate a effective culture method of osteoclasts induced by bone marrow mesenchymal stem cells. Methods:Femurs and humerus were dissected and bone marrow cells were collected and cultured from 4-week-mice.Two culture methods according to the medium with macrophage colony-stimulating factor(M-CSF) at 25 ng/ml and receptor activator of nuclear factor-κB ligand(RANKL) at 25 ng/ml or not,were compared.The tartrate-resistant acid phosphatase(TRAP) positive multineucleated and bone resorption pits were counted,the pits of bone resorption was observed by scaning electronmicroscopy.Results:TRAP positive multinuclear cells were induced from mice bone marrow mesenchymal stem cells with both M-CSF and RANKL,and these cells formed resorption pits on bone slices.The M-CSF and RANKL had positive effect on growth of osteoclasts and bone resorption pits.Conclusion:The culture system of osteoclasts induced from bone marrow mesenchymal stem cells in the presence of M-CSF and RANKL was availabe established.