Effects of Rubus parviflolius on neuronal apoptosis and expression of apoptotsis related protein in rat after local cerebral ischemic-reperfusion

Objective: To explore the mechanism of nerve-protecting effects of Rubus parviflolius(RP) on ischemic cerebral injury. Method: The middle cerebral artery was occluded for 2 h to result in the focal ischemic followed by reperfusion of 24 h.TUNEL labeling and immunohistochemical methods were used to investigate changes of neuronal apoptosis and expression of the apoptosis-related proteins after administration of RP.Result: RP decreased the apoptosis of neural cells,the expression of Bax positive cells in the RP groups significantly was decreased as compare with the control group(P0.05,P0.01);however,the expression of Bcl-2 positive cells was increased more significantly as compare with the control group(P0.05,P0.01);Bcl-2/Bax expression ratio was increased more significantly as compare with the control group(P0.05,P0.01).Conclusion: RP has action of resisting neuronal apoptosis after cerebral ischemic-reperfusion.the mechanism of nerve-protecting may be increased Bcl-2 expression,decreased Bax expression and increased Bcl-2/Bax expression ratio.