Effects of total glucosides of Rubus parviflolius L(TGRP) on neuronal apoptosis and expression of apoptosis related protein Bcl-2 and Bax in rat after local cerebral ischemic-reperfusion

Aim To explore the mechanism of neuro-protecting effects of TGRP on ischemic cerebral injury.Methods The middle cerebral artery was occluded for 2 h to produce focal ischemic followed by 24 h reperfusion.HE staining,TUNEL labeling and immunohistochemical methods were used to investigate changes of neuronal apoptosis and expression of the apoptosis-related proteins after administration of TGRP.Result TGRP decreased the pathologic changes significantly.The apoptosis of neural cells and the expression of Bax positive cells in the TGRP groups significantly decreased compared with the control group(P0.05,P0.01).However,the expression of Bcl-2 positive cells increased significantly compared with the control group(P0.05,P0.01).Bcl-2/Bax expression ratio increased significantly compared with the control group(P0.05,P0.01).Conclusion TGRP has action of resisting neuronal apoptosis after cerebral ischemic-reperfusion.The mechanism of neuroprotection may be increased Bcl-2 expression,decreased Bax expression and increased Bcl-2/Bax expression ratio.