Inhibitory effect of ~(131)I-monoclonal antibody on subcutaneously transplanted human ovarian cancer cell line OC-3-VGH tumor

OBJECTIVE: To explore the inhibitory effect of ~(131)Ⅰ-monoclonal antibody on tumor growth of human ovarian cancer cell line OC-3-VGH in nude mice. METHODS:The transplanted human ovarian carcinoma model was established by subcutaneous injection of OC-3-VGH cells in nude mice. The mice were randomly divided into negative control group,CP positive control group (60 mg/kg),high dose (10 mg/kg),low dose (2 mg/kg) group of monoclonal antibody,high dose (10 mg/kg+125 μCi),medium dose (6 mg/kg+75 μCi),low dose (2 mg/kg+25 μ 131 Ci) group of I- monoclonal antibody. Seven groups received continuously intraperitoneal injection for 14 d. Diameters of tumors were measured and nude mice were weighed on d0,d4,d8,d12,d15. The animals were killed 24 h after the last treatment. The transplanted tumors were weighed,the inhibitory rate and treatment over control growth ratios were 131 calculated. RESULTS:The high dose monoclonal antibody group,and medium and high dose I-monoclonal antibody groups had significant tumor inhibiting effects in vivo,with treatment over control growth ratios of 54%,48%,30% and inhibitory rates of 33.59%,45.80% and 64.89%,respectively. Compared with the negative control,there was significant 131 difference(P0.01) . The high dose monoclonal antibody group was compared with the high dose ~(131)Ⅰ-monoclonal antibody 131 group,showing a statistical difference(P0.05). CONCLUSION:Both monoclonal antibody and ~(131)Ⅰ- monoclonal antibody groups effectively inhibited the growth of human ovarian carcinoma in vivo. The therapeutic efficiency was 131 enhanced significantly in the high dose ~(131)Ⅰ-monoclonal antibody group.