Preparation of Meningococcal Polysaccharide Vaccine Groups A,C,W_(135) and Y

Objective To prepare meningococcal polysaccharide vaccine groups A,C,W135 and Y and remove bacterial endotoxin by a novel method.Methods Culture meningococcus,then extract and purify capsular polysaccharide groups A,C,W135 and Y.After the bacterial endotoxin was removed by ultracentrafugation and affinity chromatography,the bulks of polysaccharide groups A,C,W135 and Y were mixed according to a certain ratio,then added with stabilizer and lyophilized to prepare tetravalent meningococcal polysaccharide vaccine.Perform control tests on the vaccine according to Chinese Requirements for Biologics(2000 Edition) and the requirements in Chinese Pharmacopoeia(2005 Edition).Results All the indexes of prepared tetravalent meningococcal polysaccharide vaccine met the requirements for quality control.The vaccine was safe and effective,and its endotoxin content was not more than 10 EU/μg.Conclusion Meningococcal polysaccharide vaccine groups A,C,W135 and Y was successfully prepared.