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Expression of Grass Carp Growth Hormone in Pichia pastoris

Meng Ya-peng, Zhangfu Long

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Abstract

The ORF of grass carp growth hormone(cGH) was isolated and cloned from pGEX4T-1-cGH plasmid and inserted into pPIC9K,expression vector.The Pichia pastoris yeasts strain GS115 were transformed by electrophoration with pPIC9K-cGH and were screened on medium with G418.Colony PCR results showed that CGH gene had been integrated into the yeast chromosomes.By analyses of the SDS-PAGE and Western blotting,it was showed that cGH protein was expressed in Pichia pastoris GS115.

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What this paper is about

The ORF of grass carp growth hormone(cGH) was isolated and cloned from pGEX4T-1-cGH plasmid and inserted into pPIC9K,expression vector.The Pichia pastoris yeasts strain GS115 were transformed by electrophoration with pPIC9K-cGH and were screened on medium with G418.Colony PCR results showed that CGH gene had been integrated into the yeast chromosomes.By analyses of the SDS-PAGE and Western blotting,it was showed that cGH protein was expressed in Pichia pastoris GS115.

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Available abstract

The ORF of grass carp growth hormone(cGH) was isolated and cloned from pGEX4T-1-cGH plasmid and inserted into pPIC9K,expression vector.The Pichia pastoris yeasts strain GS115 were transformed by electrophoration with pPIC9K-cGH and were screened on medium with G418.Colony PCR results showed that CGH gene had been integrated into the yeast chromosomes.By analyses of the SDS-PAGE and Western blotting,it was showed that cGH protein was expressed in Pichia pastoris GS115.

Key concepts: Pichia pastoris, Plasmid, Grass carp, Yeast, Biology, Gene, Pichia, Expression vector

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