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Expression of Radish Peroxidase Gene RsPrx1 in Pichia pastoris and Studies on Its Tolerance

Linsong Wang

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Abstract

RsPrx1 cDNA was amplified by reverse transcription-polymerase chain reaction(RT-PCR)from the peel of Chinese red radish(Raphanus sativus L.) root.The full-length of RsPrx1 cDNA was sequenced.Then it was sub-cloned into Pichia pastoris expression plasmid pPIC9KH.RsPrx1 gene was integrated into the genome of Pichia pastoris and successfully expressed by inducing.The recombinants could show certain resistance to NaCl and H_2O_2.An efficient expression system of Pichia pastoris was constructed,which was the base of large scale production and functional analysis.

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What this paper is about

RsPrx1 cDNA was amplified by reverse transcription-polymerase chain reaction(RT-PCR)from the peel of Chinese red radish(Raphanus sativus L.) root.The full-length of RsPrx1 cDNA was sequenced.Then it was sub-cloned into Pichia pastoris expression plasmid pPIC9KH.RsPrx1 gene was integrated into the genome of Pichia pastoris and successfully expressed by inducing.The recombinants could show certain resistance to NaCl and H_2O_2.An efficient expression system of Pichia pastoris was constructed,which was the base of large scale production and functional analysis.

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Available abstract

RsPrx1 cDNA was amplified by reverse transcription-polymerase chain reaction(RT-PCR)from the peel of Chinese red radish(Raphanus sativus L.) root.The full-length of RsPrx1 cDNA was sequenced.Then it was sub-cloned into Pichia pastoris expression plasmid pPIC9KH.RsPrx1 gene was integrated into the genome of Pichia pastoris and successfully expressed by inducing.The recombinants could show certain resistance to NaCl and H_2O_2.An efficient expression system of Pichia pastoris was constructed,which was the base of large scale production and functional analysis.

Key concepts: Pichia pastoris, Raphanus, Complementary DNA, Gene, Biology, Reverse transcriptase, Gene expression, Plasmid

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