Breeding of Genetic Male Sterile Line in Greens Komatsuna

The study was done in order to solve the crosses breeding problem in utilizing hybrid vigor of Greens Komatsuna. A directional transfer program was designed by using multiple allele inherited genetic male-sterile line 06SX110 as source of male sterility, and recurrent backcrossing was employed to transfer botanical traits, while the genotype was identified through test cross.Following the procedure, the male sterile gene was transferred to the target parent Y05. Results showed that a new male sterile line GMS4 with 100% male sterility was bred. GMS4 as the female parent, the excellent hybridized combination was finally obtained. The male sterility and other horticultural characteristics in Greens Komatsuna could be transferred simultaneously based on the method designed in the research.