Study on breeding and utilization of genetic male sterile line in dwarf Chinese cabbage.
Hao Wang, Ning Yang, Hui Feng
Abstract
Hao Wang, Ning Yang, Hui Feng
Abstract
A directional transfer program was designed by using multiple allele inherited genetic male-sterile line AB01 and its temporary maintainer line B01 as source of male sterility,and recurrent backcrossing was employed to transfer botanical traits,while the genotype was identified through test cross.Following the procedure,the male sterile gene was successfully transferred to the target parent Y,the inbred line of Jinguan in dwarf Chinese cabbage.A new male sterile line GMS401,which is similar to Y on botanical traits with 100% male sterility and 100% male sterile plants,was bred.The results showed that GMS401 as the female parent,the hybridized combinations GMS401×D1 and GMS401×D2 were finally obtained and they were excellent in horticultural characters and uniformity.In addition,the yield of the combinations was apparently higher than that of CK.
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A directional transfer program was designed by using multiple allele inherited genetic male-sterile line AB01 and its temporary maintainer line B01 as source of male sterility,and recurrent backcrossing was employed to transfer botanical traits,while the genotype was identified through test cross.Following the procedure,the male sterile gene was successfully transferred to the target parent Y,the inbred line of Jinguan in dwarf Chinese cabbage.A new male sterile line GMS401,which is similar to Y on botanical traits with 100% male sterility and 100% male sterile plants,was bred.The results showed that GMS401 as the female parent,the hybridized combinations GMS401×D1 and GMS401×D2 were finally obtained and they were excellent in horticultural characters and uniformity.In addition,the yield of the combinations was apparently higher than that of CK.
Key concepts: Backcrossing, Sterility, Biology, Allele, Genetics, Software maintainer, Genotype, Horticulture