Effects of picloram on tulip callus induction and somatic embryogenesis

The effects of 3 reagents such as picloram on tulip callus induction and somatic embryogenesis were studied by using the optimal design of311-Bregression.The results showed that the callus inductivity and the somatic embryogenesis had a very significant correlation with picloram concentration.Picloram played a decisive role in tulip callus induction,and the callus could occur through 20 d culture.The optimal medium was MS+ 6 mg/L picloram+ 0.5 mg/L BA,the callus inductivity reached 100%and each piece of the callus averagely produced 10 embryoids.Picloram and BA had a positive interaction and could be used together.But picloram had a negative interaction with hymexazol and both did not have to be used together.