Effect of Picloram on Paeonia Callus Induction and Somatic Embryogenesis

The material was the cultivar 'Fengdan'of Paeonia suffruticosa.,immature embryos,mature embryos,petioles and roots of which were used as explants to induced calli.Effects of picloram on Paeonia callus induction and somatic embryogenesis were studied.The result showed that callus were produced from all kind of explants,but only the immature embryos and mature embryos could produce the embryogenic calli.Somatic embryos were induced from the embryogenic callus by subculturing and regenerated plantlets were obtained.The optimum media of callus induction and somatic embryos production were the modified MS+BA 0.5mg·L-1 +PIC 4.0mg·L-1+CH 0.5mg·L-1+ Sucrose 100g·L-1,callus induction rate was 99.3%,and embryos inductivity was 98.1%.