RP-HPLC simultaneous determination of chlorogenic acid and caffeic acid in Wuweixiaoduyin oral solution

Objective To establish an RP-HPLC method for simulaneous determination of chlorogenic acid and caffeic acid in Wuweixiaoduyin oral solution.Methods Irregular-H C18 column(4.6 mm×250 mm,10 μm) was used with acetonitrile-0.04% phosphoric acid solution(V∶V=12∶88) as the mobile phase at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 327 nm and column temperature was 25 ℃.Results The calibration curve was linear in the range of 4.08-40.8 mg·L-1(r=0.999 6)and 0.156-1.56 mg·L-1(r=0.999 8)for chlorogenic acid and caffeic acid,respectively.The average recoveries were 99.7% and 99.3% with RSD of 0.52% and 0.27%(n=9),respectively.Conclusions The method is simple,accurate and reliable,which may be used for simultaneous determination of chlorogenic acid and caffeic acid in Wuweixiaoduyin oral solution.