Simultaneous Determination of Chlorogenic Acid and Caffeic Acid of Compound Puqin Tablets by HPLC

Objective To establish a method for the determination of chlorogenic acid and caffeic acid of compound puqin tablets. Methods HPLC method was used with Agilent ZORBAX C18(250mm×4.6mm, 5μm)column and the mobile phase consisted of acetonitrile-0.02mol/L NaH2PO4(19:81). The flow rate was 1.0mL?min-1,the column temperature was 30℃,and the UV detector was set at 323nm. Results The linear ranges of chlorogenic acid and caffeic acid were 0.007~0.148mg?mL-1(r=0.999 8)and 0.007~0.132mg?mL-1(r=0.999 9), respectively.The average recovery of chlorogenic acid and caffeic acid were 98.2% and 98.7%, respectively. Conclusion The method is simple, repeatable and accurate, it can be applied in quantitative determination of chlorogenic acid and caffeic acid in compound puqin tablets.