Study on culture medium optimization of phosphorus and potassium dissolving Bacillus mucilaginosus

The main influence factors,including carbon sources,nitrogen source,which influence the bacterial growth,was optimized through single factor;Orthogonal experiment was used to optimize the additive quality of carbon sources and nitrogen source,and analyze the maximum impact factor of the bacterial biomass and the optimal combination of culture media components.The results showed that:by single factor experiment we can know there is higher bacteria biomass when glucose was used for carbon source,and ammonium sulfate was used for nitrogen source.The results of orthogonal experiment showed that glucose was the main impact factors of the bacterial biomass;the optimization of the ideal culture medium formula is(g /L) :glucose 24,ammonium sulfate 1,DouBing 9,calcium carbonate 2,magnesium sulfate 2,ammonium sulfate 2,potassium dihydrogen phosphate 4,ferric chloride 0.1.Under this condition,the living bacterium number can reach 8 × 108 cfu /mL.