Resveratrol induced apoptosis of U-87 glioma cells through the Fas-mitochondria pathway

Objective To investigate the proliferation-inhibiting and apoptosis-inducing activity of resveratrol(Res)on human brain glioma cell line U-87 in vitro and the possible molecular mechanisms.MethodsHuman brain glioma cell line U-87 was used as the target cells to detect the cell proliferating activity by a methyl thiazolyl tetrazolium(MTT)colorimetric assay.The apoptosis of U-87 cells was assessed with FITC-Annexin V and propidium iodide(PI)double staining and the morphological observation.Expression of Fas protein,mitochondrial inner membrane potential(Δψm),cytochrome c(Cyt c)release,and caspase-3 activity were analyzed by flow cytometry(FCM).Results Res significantly inhibited the proliferation of U-87 cells in a dose-and time-dependent manner(P0.01).After treated with 20 mol/L and 40 mol/L of Res,the apoptosis rate of U-87 cells detected Annexin V/PI staining obviously increased to 42.57%~62%,and the typical apoptotic morphological changes of U-87 cells were observed.FCM detected the expression of Fas protein increased by 1.6~2.2 times,accompanied with a decrease in mitochondria Δψm by 15%~63% and the increased release of Cyt c by 2.5~7.3 times from mitochondria to cytoplasm.The activity of caspase-3 was significantly enhanced by 25%~112%.Conclusion Res greatly inhibits the proliferation of U-87 cells and induces the apoptosis of U-87 cells via the Fas-mitochondria pathway in vitro.