MnSOD_m induces apoptosis in leukemia K562 cells through Fas-dependent pathway

AIM:To investigate the apoptosis of human leukemia cell line K562 induced by mimics of manganese superoxide dismutase(MnSODm)in vitro and the possible molecular mechanisms.METHODS:The apoptosis in K562 cells was examined by MTT colorimetric method,FITC-Annexin V and propidium iodide(PI)double staining and morphological changes method,the expression level of Fas protein was measured with flow cytometry(FCM),and the mRNA expression and the activity of Caspase-3 were detected by RT-PCR and colorimetric assay,respectively.RESULTS:After administration with MnSODm,the proliferation of K562 cells was obviously inhibited,the cellular apoptosis was markedly enhanced with Annexin V/PI staining and the cells showed the typical apoptotic morphological changes.The expression of Fas protein in K562 cells up-regulated greatly,which accompanied with the significant increase of both mRNA expression and activity of Caspase-3.CONCLUSION:The apoptosis of leukemia K562 cells induced by MnSODm may involved in the Fas death receptor pathway.