Inhibitory Effects of Sulforaphen on Starvation-induced Apoptosis of Endothelial Cells

OBJECTIVE:To study the inhibitory effects of sulforaphen on starvation-induced apoptosis of endothelial cells.METHODS:The apoptosis of HUVECs was induced by starvation culture method. The apoptotic HUVECs were randomly divided into normal control group(complete culture solution),model group(model culture solution)and sulforaphen high-concentration and low-concentration groups(10,5 μmol/L). The morphology of HUVECs was observed,and the survival rate of HUVECs was detected by SRB method. The apoptosis of HUVECs was observed by Hoechst 33258 staining. The change of HUVECs autophagy was observed by acridine orange staining and Western blot assay. RESULTS:The sulforaphen could inhibit the phenomena that the edge of HUVECs became shiny and isolated from vessel bottom as time went on,enhanced the survival rate of HUVECs;inhibited the apoptosis of HUVECs in dose and time-dependant manner;improved HUVECs autophagy. CONCLUSIONS:The sulforaphen can inhibit the apoptosis of HUVECs by inducing HUVECs autophagy.