Effects of TSG on Apoptosis of HUVECs Induced by H_2O_2

Objective: To investigate the protective effect of 2,3,5,4'-tetrahydroxystilbene-2- O-β -D-glucoside (TSG on human umbilical vein endothelial cells (HUVECs) apoptosis induced by H2O2. Methods: HUVECs were treated with H2O2 (100,200,300,400,500μmol/L) for 24 hours, the optimal concentration of H2O2 was selected by MTT and Flow Cytometry. HUVECs weretreatedwithTSG (0.1,1,10,100μmol/L)for24hoursthenexposedtoH2O2 (100,200,300,400,500μmol/L)for24 hours, cellviability rates and apoptosis rates of different concentration of TSG pretreatment group were detected by MTT and Flow Cytometry. Mophology of apoptosis and the protective effect of TSG on HUVECs induced by H2O2 were detected by Hoechst33258 staining. Results: The opti- mal concentration for H2O2 to establish apoptosis model were 300μmol/L. Cell viability rate of TSG pretreatment group (10μmol/L and 100μmol/L) increased (P0.05) while the apoptosis rate de- creased obviously (P0.01) compared with H2O2 group(300μmol/L). TSG reduced the number of HUVECs apoptosis. Conclusions:TSG may have a protective effect on HUVECs apoptosis induced by H2O2.