Cloning and sequenceing of HN gene of avian paramyxovirus strain NA-1 isolated from goose

Avian paramyxovirus strain NA-1 isolated from goose was propagated in 11-day-old chicken embryos.The genomic RNA of NA-1 were extracted.The HN gene of the virus has been successfully amplified by RT-PCR,and further cloned into pMD 18-T vector,and a positive recombinant plasmid was obtained by restriction enzyme analysis. The complete sequence of HN gene was obtained finally and the nucleotide sequence of the HN gene was 1 740 bp.The sequence analysis demonstrated that 15 reference strains loaded down from GenBank,the result of sequence analysis indicated that NA-1 strain exhibited 97.3?% homology with reference strain YG 97,84.5?% homology with strain F-(48)E-9 and 82.2?% homology with strain La Sota. The results indicated the mutation of the HN gene between the goose's paramyxovirus and NDV varied to a great extent.