ScholarLens Back to search
2002•Zhongguo shouyi ke-jiRequires access

Cloning and sequencing of HN gene of a goose paramyxovirus isolate

Zhao Wen-hua

Open publisher page 2 citations

Abstract

Virion RNA was abstracted from purified GPV-SF02 strain and used as a template. The fragment of the hemagglutinin-neuriminidase gene of the isolate was amplified by RT-PCR and ligated with the pMD 18-T Vector. Positive clones of HN gene were identified by PCR and then sequenced. The complete sequence of HN gene was obtained finally. The result of sequence analysis for the ORF of HN gene indicats that GPV-SF02 strain exhibited 86% homology with reference strain NL-96.

About this research paper

What this paper is about

Virion RNA was abstracted from purified GPV-SF02 strain and used as a template. The fragment of the hemagglutinin-neuriminidase gene of the isolate was amplified by RT-PCR and ligated with the pMD 18-T Vector. Positive clones of HN gene were identified by PCR and then sequenced. The complete sequence of HN gene was obtained finally. The result of sequence analysis for the ORF of HN gene indicats that GPV-SF02 strain exhibited 86% homology with reference strain NL-96.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Virion RNA was abstracted from purified GPV-SF02 strain and used as a template. The fragment of the hemagglutinin-neuriminidase gene of the isolate was amplified by RT-PCR and ligated with the pMD 18-T Vector. Positive clones of HN gene were identified by PCR and then sequenced. The complete sequence of HN gene was obtained finally. The result of sequence analysis for the ORF of HN gene indicats that GPV-SF02 strain exhibited 86% homology with reference strain NL-96.

Key concepts: Biology, Gene, Homology (biology), Cloning (programming), Virology, Sequence analysis, Hemagglutinin (influenza), Goose

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and sequencing of HN gene of a goose paramyxovirus isolate — Research Paper | ScholarLens