Effects of adipocytes on osteogenic potential of bone marrow stromal cell

Objective To investigate the effects of adipocyte in bone marrow cavity on osteogenic potential,and study the pathogenic mechanism of adipocyte differentiation from bone marrow stromal cells in osteoporosis.Methods Bone marrow stromal cells were inoculated in culture plate with the concentration of 105/ml and divided into 5 groups,which were added with adipocytes with concentrations of 0,103,104,105,106/ml respectively.The co-culture system of adipocyte and bone marrow stromal cell was established.After 4,8,12 days,the samples were detected for the activity of alkaline phophatase,the mRNA expression of type I collagen by in situ hybrization,the collagen synthesis ability with 3H-proline in corporation test.[WT5”HZ]Results After 12 days,with the increase of concentration of adipocytes,the activity of alkaline phophatase was significantly different between experimental groups and control (P 0.05 or P 0.01).The mRNA expression level of type I collagen in control group was significantly higher than in other groups,and collagen synthesis ability was decreased in the experimental group.Conclusion Adipocytes in bone marrow cavity can inhabite osteogenic potential of bone marrow stromal cells.It might play an important role in osteoporosis.