Experimental Study of Oriental Adipogenesis of Bone Marrow Stromal Cells

Objectives To observe steriod - induced bone marrow stromal cell differentiation into adipocyte and to provide some adipocytes for the latter experiments.Methods The primary bone marrow stromal cells of the femur of the rat were procured and expanded in culture medium. The samples were treated with dexamethasone which acted as adipocyte differentiation inducer. The cultured cells were stained with Oil Red O. The number of adipocytes were counted under a light microscope.Results After the stromal cells were continuously cultured in the culture fluid containing different (0, 1×10-8,1×10-7,1×10-6 and 1×10-5mol/L) concentrations of dexamethasone for 21 days, the number of adipocytes, which was highest in 1×10-5mol/L group and lowest in the control group, increased with higher concentration of dexamethasone and was a dose - dependent. Conclusions Dexamethasone can directly induce bone marrow stromal cell differentiation into adipocytes. It might be one of causes of steriod - induced osteoporosis.