Modification of Alkaline Lysis Method for Preparation of Plasmid DNAs from Recombinant E.coli

In order to decrease the cost of the plasmid production,the standard alkaline lysis method for preparation of plasmid DNAs was modified,including the use of optimized volume of solution I to resuspend the centrifuged bacteria and a new solution III which contain less KAc to neutralize the cell lysate.Purification and quantitative analysis by BCA detection,Real-time PCR and agrose gel electrophoresis showed that both yield and quality of plasmid DNA prepared by the modified alkaline lysis remain unchanged compared to that by standard alkaline lysis method,but the cost was decreased to about 1/4 of normal method,which laid a solid foundation for large-scale production of recombinant plasmid DNAs for animal use.