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A comparative and improved study on plasmid DNA extraction

Dawei Guo

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Abstract

Objective To explore a simple,improved and steady method for extraction of plasmid DNA and its value in molecular biological research. Methods Four routine DNA extractions of plasmid were compared by using agarose gel electrophoresis. Results The band using TIANprep Mini Plasmid Kit was the brightest without RNA band.The brightness of the bands increased using boiling lysis method,alkaline lysis method,alkaline lysis method combined with phenol-chloroform extraction method in turns. Conclusion Lysis by TIANprep Mini Plasmid Kit is simple and convenient for producing a high purity of plasmid DNA.

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Objective To explore a simple,improved and steady method for extraction of plasmid DNA and its value in molecular biological research. Methods Four routine DNA extractions of plasmid were compared by using agarose gel electrophoresis. Results The band using TIANprep Mini Plasmid Kit was the brightest without RNA band.The brightness of the bands increased using boiling lysis method,alkaline lysis method,alkaline lysis method combined with phenol-chloroform extraction method in turns. Conclusion Lysis by TIANprep Mini Plasmid Kit is simple and convenient for producing a high purity of plasmid DNA.

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Available abstract

Objective To explore a simple,improved and steady method for extraction of plasmid DNA and its value in molecular biological research. Methods Four routine DNA extractions of plasmid were compared by using agarose gel electrophoresis. Results The band using TIANprep Mini Plasmid Kit was the brightest without RNA band.The brightness of the bands increased using boiling lysis method,alkaline lysis method,alkaline lysis method combined with phenol-chloroform extraction method in turns. Conclusion Lysis by TIANprep Mini Plasmid Kit is simple and convenient for producing a high purity of plasmid DNA.

Key concepts: Alkaline lysis, Lysis, Plasmid, Plasmid preparation, Agarose, DNA, DNA extraction, Agarose gel electrophoresis

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