The extraction process of geniposide optimized by orthogonal experiment

Objective: To optimize extraction process of geniposide in gardenia.Methods: Orthogonal experimental method was used to the extraction of the geniposide,high performance liquid chromatography(HPLC) method were used to determine the content of geniposide of extract,and variance analysis to determine the best gardenia extracting process.Results: The best extraction process of geniposide should meet the following requirements: ethanol concentration is 80%,10 times of the amount of alcohol,1.5 hours extraction time,2 times for each process. In this process,the average content of validation geniposide is 6.1854%. Conclusion: The extract method of gardenia is simple and easy,and it is reliable for quality control.