Effect of Inoculation Method on Toxicity of Beauveria bassiana against 5~(th) Instar Larvae of Homona coffearia
WU Guang-yuan
Abstract
WU Guang-yuan
Abstract
Toxicities of Beauveria bassiana strains,JYBb201 and JYBb202,against the 5th instar larvae of Homona coffearia were compared when 4 different methods of inoculation were applied.The adjusted mortality rates of H.coffearia larvae subjected to 1.0×107 conidia·mL-1 spore suspensions of JYBb201 and JYBb202 were found to be 85.00% and 81.94%,respectively,when the larvae were submerged in the spore suspension;82.50% and 76.94%,respectively,when the larvae were smeared with the spore suspension;47.50% and 43.61%,respectively,when the tea tips were dipped in the suspension;and,52.50% and 48.61%,respectively,when the spore suspension was sprayed on the larvae.The immersing method also appeared to bring about the highest cadaver rates at 67.50% for JYBb201 and 57.50% for JYBb202.It was the same when the LT50 values were used for the comparison 3.510 d for JYBb201 and 3.808 d for JYBb202.
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Toxicities of Beauveria bassiana strains,JYBb201 and JYBb202,against the 5th instar larvae of Homona coffearia were compared when 4 different methods of inoculation were applied.The adjusted mortality rates of H.coffearia larvae subjected to 1.0×107 conidia·mL-1 spore suspensions of JYBb201 and JYBb202 were found to be 85.00% and 81.94%,respectively,when the larvae were submerged in the spore suspension;82.50% and 76.94%,respectively,when the larvae were smeared with the spore suspension;47.50% and 43.61%,respectively,when the tea tips were dipped in the suspension;and,52.50% and 48.61%,respectively,when the spore suspension was sprayed on the larvae.The immersing method also appeared to bring about the highest cadaver rates at 67.50% for JYBb201 and 57.50% for JYBb202.It was the same when the LT50 values were used for the comparison 3.510 d for JYBb201 and 3.808 d for JYBb202.
Key concepts: Beauveria bassiana, Spore, Instar, Inoculation, Larva, Biology, Bassiana, Conidium