Influence of Li's Prescription I for Asthma on Apoptosis of Eosinophils and Expression of Bcl-2 Gene in Asthmatic Mice

Objective To study the influence of Li's Prescription Ⅰfor Asthma(LPAⅠ)on apoptosis of eosnophils(EOS)in lung tissue of asthmatic mice,and to explore its possible therapeutic mechanism for asthma.Methods Sixty Kunming male mice with specific pathogen free were equally randomized into 6 groups:normal group,model group,dexamethasone group(2mg/kg),and low-,middle-and high-dosage LPAⅠ groups(in the dose of 6.4,12.8 and 19.2 g·kg-1·d-1,respectively).Mice models of bronchial asthma were induced by sensitization with intraperitoneal injection of ovalbumin(OVA)and challenge with nasal inhalation of OVA.The expression of apoptosis gene and anti-apoptosis gene bcl-2 in EOS of mice lung tissue was detected with in situ DNA end-labeling technique and immunohistochemical assay.Results EOS apoptotic index was higher(P0.01),and bcl-2 expression level in EOS was lower(P0.05 or P0.01)in LPAⅠ groups than those in the model group.Conclusion The therapeutic mechanism of LPAⅠ for bronchial asthma is possibly related with inhibiting bcl-2 expression,promoting apoptosis of EOS and reducing hyperreactive inflammation in lung tissue of asthmatic mice.