Optimization of Extraction Process for β-1,3-Glucan from Yeast Paste by Response Surface Methodology

In order to improve the extraction rate of β-1,3-glucan from yeast paste,response surface methodology was used for the optimization of alkaline extraction conditions.The effects of NaOH concentration,extraction time and extraction temperature on the extraction rate of β-1,3-glucan were explored by single factor tests.Subsequently,a regression model was established using a central composite design.Based on the model,response surface analysis was conducted to explore optimal extraction conditions.The results indicated that the optimal extraction process parameters were NaOH concentration of 2.05%,extraction temperature of 92.70 ℃ and extraction time of 2.50 h.Under these optimal extraction conditions,the extraction rate of β-1,3-glucan was up to 15.76%,which was consistent with the predicted value of 15.82%.Therefore,the extraction rate of β-1,3-glucan were enhanced by response surface methodology.