2012•Science and Technology of Food IndustryRequires access

Optimization of extraction technology of β-glucan from barely by response surface methodology

Guohua Zhao

Abstract

On the basis of single-factor tests,ethanol reflux time,water extraction time,water extraction temperature,ratio of ethanol volume effect extraction rate of β-glucan from barely were optimized using Box-Behnken center composite design and response surface methodology(RSM).The results showed that the optimum ethanol reflux time,water extraction time,water extraction temperature,ratio of ethanol volume were 3 hours,2.5 hours,85℃ and 5 in respectively.Under these conditions,the extraction rate was 7.75% while the predicted one was 7.81%.

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Key concepts: Response surface methodology, Extraction (chemistry), Chromatography, Ethanol, Volume (thermodynamics), Water extraction, Surface-area-to-volume ratio, Chemistry