Detection and typing of human papilloma viruses in juvenile pharynllaryngeal papillomatosis.

Objective:To study the relationship between infection of human papilloma virus and juvenile pharynlaryngeal papilloma. Methods:Polymerase chain reaction (PCR) and dot blot hybridization were used to detect HpV 6、11、16、18、33 DNA in 35 samples of pharynlaryngeal papilloma and 10 samples of .vocol nodule. Results:The positive rate of HPV in pharynlaryngeal laryngeed papilloma was 91.4% (30/35) . The positive rate of HPV 6 and HPV 1 1 were 54.2% (19/35) and 25.7% (9/35). The positive rate of multiple types of HPV 6+11 was 11.4% (4/35).The positive rate of HPV 16、18、33 were not significant. The positive rate of HPV in vocol nodule was not significant .Conclusion:It is suggested that infection with HPV,especially with HPV 6 is closely associated with the development of juvenile pharynlaryngeal larynged papilloma in Wenzhou area. [FK(WB80011。5]