[Human papillomavirus-DNA in laryngeal papilloma detected by nucleic acid hybridization and the clinical treatment].

We examined 16 recurrent respiratory papillomatosis patients, who were undergoing treatment for recurrence of their diseases, for the presence of human papilloma virus (HPV). Biopsies were obtained from the papillomas site of larynx or trachea. The HPV-DNA 6b, 11, 16 were determined by using Slot blot hybridization technique, type HPV-6b positive 87.5%, HPV-11 positive 93.7% HPV-16 positive 81.6%. Using Southern blot procedure examined 12 HPV-DNA positive samples, the results show that 3 were typical HPV-11.5 were undetermined types. All cases were treated by endoscopic or microendoscopic surgery repeatedly and followed up for 2-10 years. The remission rate was 71% (10/14).