Simultaneous determination of the contents of saponins in compound Shougong powder by UPLC

OBJECTIVE To establish an UPLC method for the determination of notoginsenoside R1,ginsenosides Rg1,ginsenosides Re and ginsenoside Rb1in compound Shougong powder.METHODS The determination was performed on Acquity BEH C18(2.1 mm×100 mm,1.7μm)with mobile phase consisted of acetonitrile-water(gradient elution)at a flow rate of 0.25ml/min.Column temperature was 26℃.The UV detection wavelength was set at 203 nm.RESULTS The calibration curves were linear in the range of 0.053~0.53μg(r=0.999 8)for notoginsenoside R1,0.067 5~0.675μg(r=0.999 8)for ginsenosides Rg1,0.061~0.61μg(r=0.999 6)for ginsenosides Re,0.066 3~0.662 5μg(r=0.999 8)for ginsenosides Rb1.The average recovery was 97.96%,97.81%,97.18%,98.52% and RSD was 1.69%,1.85%,2.73%,2.00% respectively.CONCLUSION The determination method is fast,reliable,accurate,and it can be used for quality control of compound Shougong powder.